The effect of dietary lipids on microsomal electron transport proteins in the rat

Feeding a fat-free diet for 3 weeks decreases the activity of ascorbate:ferricytochrome b5 oxidoreductase and increases NADH:monodehydroascorbate oxidoreductase in rat liver microsomes. Contrary to Vmax, the apparent Km for monodehydroascorbate of the first enzyme was not depressed. The activity of...

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Veröffentlicht in:	Annals of nutrition and metabolism 1984, Vol.28 (5), p.261-267
Hauptverfasser:	Domke, I, Weis, W
Format:	Artikel
Sprache:	ger
Schlagworte:	Animals Carrier Proteins - metabolism Dietary Fats - pharmacology Electron Transport - drug effects Kinetics Male Microsomes, Liver - enzymology Microsomes, Liver - metabolism NAD - metabolism Oxidoreductases - metabolism Rats Rats, Inbred Strains
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Zusammenfassung:	Feeding a fat-free diet for 3 weeks decreases the activity of ascorbate:ferricytochrome b5 oxidoreductase and increases NADH:monodehydroascorbate oxidoreductase in rat liver microsomes. Contrary to Vmax, the apparent Km for monodehydroascorbate of the first enzyme was not depressed. The activity of ascorbate:ferricytochrome b5 oxidoreductase is increased by incubation with microsomal lipid extract, thus showing that the loss of activity results from dietary-induced changes of microsomal fatty acid composition.
ISSN:	0250-6807
DOI:	10.1159/000176815