Immobilized pronase

Immobilized pronase

Pronase was successfully coupled to an arylamino derivative of porous glass. The immobilized enzyme retains considerable activity against large and small substrates: bovine serum albumin, 57%; benzoyl-L-arginine ethylester, 27%; L-leucine-p-nitroanilide, 67%. Studies of enzyme stability, pH behavior...

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Veröffentlicht in:Biochemical and biophysical research communications 1971-07, Vol.44 (2), p.426-432
Hauptverfasser: Royer, G.P., Green, G.M.
Format: Artikel
Sprache:eng
Schlagworte:
Anilides
Animals
Arginine
Azo Compounds
Benzoates
Cattle
Chemical Phenomena
Chemistry
Drug Stability
Esters
Glass
Hydrogen-Ion Concentration
Kinetics
Leucine
Nitrobenzenes
Peptide Hydrolases
Pronase
Serum Albumin, Bovine
Spectrophotometry
Streptomyces - enzymology
Thermodynamics
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Beschreibung
Zusammenfassung:Pronase was successfully coupled to an arylamino derivative of porous glass. The immobilized enzyme retains considerable activity against large and small substrates: bovine serum albumin, 57%; benzoyl-L-arginine ethylester, 27%; L-leucine-p-nitroanilide, 67%. Studies of enzyme stability, pH behavior and temperature dependence are presented.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(71)90618-8