Identification of Atypical Strains of Herpes Simplex Virus Using a Simplified DNA Fingerprinting Method

In order to further confirm the usefulness and reliability of the authors' simplified DNA fingerprinting procedure, the genomes of 11 HSV strains giving rise to clear, equivocal or contradictory results in biological or serological tests were blindly analyzed after cleavage with a single restri...

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Veröffentlicht in:	MICROBIOLOGY and IMMUNOLOGY 1984, Vol.28(6), pp.723-729
Hauptverfasser:	Hamelin, Claude, Chagnon, André, Fauvel, Micheline
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Deoxyribonuclease EcoRI DNA Restriction Enzymes DNA, Viral - isolation & purification Fundamental and applied biological sciences. Psychology Herpes Simplex - microbiology herpes simplex virus herpes simplex virus 1 herpes simplex virus 2 Humans Microbiology Simplexvirus - classification Simplexvirus - isolation & purification Species Specificity Techniques used in virology Virology
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Beschreibung
Zusammenfassung:	In order to further confirm the usefulness and reliability of the authors' simplified DNA fingerprinting procedure, the genomes of 11 HSV strains giving rise to clear, equivocal or contradictory results in biological or serological tests were blindly analyzed after cleavage with a single restriction enzyme (EcoR I). All strains were clearly identified as either HSV-1 or HSV-2 on the basis of stable differences between specific and well-resolved DNA markers.
ISSN:	0385-5600 1348-0421
DOI:	10.1111/j.1348-0421.1984.tb00727.x