Selection of Ribosomal Mutants by Antibiotic Suppression in Yeast

Selection of Ribosomal Mutants by Antibiotic Suppression in Yeast

Wild-type Saccharomyces cerevisiae is highly resistant to streptomycin. A histidine auxotroph was found which could grow without histidine in the presence of high concentrations of streptomycin. Selection for derivatives of this strain which could be suppressed by much lower concentrations of strept...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1971-12, Vol.174 (4016), p.1339-1341
Hauptverfasser: Bayliss, Frank T., Vinopal, Robert T.
Format: Artikel
Sprache:eng
Schlagworte:
Antibiotics
Autoradiography
Bacteria
Carbon Isotopes
Cell growth
Cell separation
Creatine
Drug Resistance, Microbial
Genetic loci
Genetic mutation
Genotype
Histidine - metabolism
Mutation
Neomycin
Nitrites
Ribosomes
Ribosomes - analysis
Saccharomyces - drug effects
Saccharomyces - metabolism
Saccharomyces cerevisiae - drug effects
Streptomycin - pharmacology
Tritium
Uracil - metabolism
Yeasts
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Zusammenfassung:Wild-type Saccharomyces cerevisiae is highly resistant to streptomycin. A histidine auxotroph was found which could grow without histidine in the presence of high concentrations of streptomycin. Selection for derivatives of this strain which could be suppressed by much lower concentrations of streptomycin yielded streptomycin-sensitive mutants which are cold-sensitive and have altered ribosomal profiles.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.174.4016.1339