Peripheral-type benzodiazepine binding sites in a renal epithelial cell line (MDCK)

Madin-Darby canine kidney (MDCK) cells express a high density of binding sites (Bmax = 0.67 pmol/106 cells) for [3H]RO 5-4864, a peripheral-type benzodiazepine (BZD) receptor ligand. Receptor affinity (Kd = 45 nM) in MDCK cells is 30–50 fold lower than in rat kidney, but its pharmacological specific...

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Veröffentlicht in:	European journal of pharmacology 1984-08, Vol.103 (1-2), p.185-188
Hauptverfasser:	Beaumont, Kevin, Moberly, James B., Fanestil, Darrell D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Benzodiazepines Benzodiazepinones - metabolism Biological and medical sciences Cell Line Cell Membrane - metabolism Convulsants - metabolism Dogs Drug receptors Epithelium Epithelium - metabolism Ion Channels - metabolism Kidney - metabolism Kinetics MDCK cells Medical sciences Neuropharmacology Pharmacology. Drug treatments Psycholeptics: tranquillizer, neuroleptic Psychology. Psychoanalysis. Psychiatry Psychopharmacology Receptors, GABA-A - analysis Receptors, GABA-A - metabolism Ro 5-4864
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Zusammenfassung:	Madin-Darby canine kidney (MDCK) cells express a high density of binding sites (Bmax = 0.67 pmol/106 cells) for [3H]RO 5-4864, a peripheral-type benzodiazepine (BZD) receptor ligand. Receptor affinity (Kd = 45 nM) in MDCK cells is 30–50 fold lower than in rat kidney, but its pharmacological specificity is identical to that of peripheral-type BZD receptors in the rat kidney (PK 11195 > RO5–4864 > diazepam = flunitrazepam > clonazepam). The MDCK cell line should provide a useful model system for studying the role of peripheral type BZD receptors in renal function.
ISSN:	0014-2999 1879-0712
DOI:	10.1016/0014-2999(84)90210-3