Human Leukemic Cells: In vitro Growth of Colonies Containing the Philadelphia (Ph$^{1}$) Chromosome

Human Leukemic Cells: In vitro Growth of Colonies Containing the Philadelphia (Ph$^{1}$) Chromosome

Human leukemic cells with a marker (Philadelphia; Ph$^{1}$) chromosome gave rise to granulocytic and mononuclear cell colonies when grown in vitro. All metaphases from a single colony were either Ph$^{1}$ positive or Ph$^{1}$ negative. No colonies contained a mixed cell population. This suggests tha...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1971-12, Vol.174 (4014), p.1134-1136
Hauptverfasser: Chervenick, Paul A., Ellis, Lawrence D., Pan, Sylvia F., Lawson, Alton L.
Format: Artikel
Sprache:eng
Schlagworte:
Antiserum
Bone marrow
Bone Marrow - growth & development
Bone Marrow Cells
Cell lines
Chromosome Aberrations
Chromosomes
Chromosomes, Human, 21-22 and Y
Chronic myeloid leukemia
Clone Cells - growth & development
Cultured cells
Humans
Karyotyping
Leukemia
Leukemia, Myeloid - genetics
Leukemia, Myeloid - pathology
Leukocytes
Leukocytes - growth & development
Metaphase
Mitosis
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Zusammenfassung:Human leukemic cells with a marker (Philadelphia; Ph$^{1}$) chromosome gave rise to granulocytic and mononuclear cell colonies when grown in vitro. All metaphases from a single colony were either Ph$^{1}$ positive or Ph$^{1}$ negative. No colonies contained a mixed cell population. This suggests that leukemic and normal cells exist simultaneously and that in vitro colonies are clonal in origin.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.174.4014.1134