Renoprotective potency of amifostine in rat renal ischaemia–reperfusion

Purpose. Kidneys from haemodynamically unstable donors may suffer from renal ischaemia–reperfusion (RIR) injury. RIR is associated with reactive oxygen species production that induces inflammation and activates the arachidonic acid (AA) pathway which converts AA into prostaglandin E2. Amifostine was investigated for its renoprotective potential in RIR. Materials and methods. The effect of amifostine (25 mg/kg = 910 mg/m2) on the COX pathway, enzymatic antioxidant activity, the lipid peroxidation marker MDA, serum creatinine and apoptosis was determined in rats. Kidneys were subjected to 45 min of ischaemia and 1 or 24 h of reperfusion. Control groups (sham: coeliotomy, no ischaemia; r1: 45 min ischaemia/1 h reperfusion; r2: 45 min ischaemia/24 h reperfusion) were administered physiological saline intraperitoneally, and treated groups (E1: 45 min ischaemia/1 h reperfusion; E2: 45 min ischaemia/24 h reperfusion) received amifostine 30 min before reperfusion. Results. Serum creatinine increased in non-treated control rats: r1 vs sham (1.6-fold; P