Isolation and protein composition of normal and petit capsids of bacteriophage lambda

Isolation and protein composition of normal and petit capsids of bacteriophage lambda

Normal and petit lambda capsids, prepared from lysates of Escherichia coli W3350 (λ Sus M87), were separated from one another by chromatography on ECTEOLA cellulose. Each capsid type was degraded chemically to its protein subunits by treatment with sodium dodecyl sulfate at alkaline pH, and its comp...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1971-11, Vol.46 (2), p.298-309
Hauptverfasser: Huntley, G.H., Kemp, C.L.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acids - analysis
Autoanalysis
Chromatography, Ion Exchange
Coliphages - analysis
Densitometry
Electrophoresis, Disc
Escherichia coli
Hydrogen-Ion Concentration
Lysogeny
Microscopy, Electron
Molecular Weight
Phosphotungstic Acid
Potassium
Sodium
Staining and Labeling
Sulfates
Viral Proteins - analysis
Viral Proteins - isolation & purification
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Zusammenfassung:Normal and petit lambda capsids, prepared from lysates of Escherichia coli W3350 (λ Sus M87), were separated from one another by chromatography on ECTEOLA cellulose. Each capsid type was degraded chemically to its protein subunits by treatment with sodium dodecyl sulfate at alkaline pH, and its components were analyzed by electrophoresis in acrylamide gels containing SDS. The normal lambda capsids, free from DNA, contained two proteins of molecular weight 45,000 and 14,000. Petit lambda was composed mainly of the same 45,000 molecular weight protein as normal lambda, but totally lacked the low molecular weight component. The amino acid composition of the two capsid types and their components was determined.
ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(71)90031-6