4-Methylumbelliferyl-glycosides as fluorescence probes of sugar-binding sites on lectin molecules: Spectral properties and dependence of fluorescence on polarity and viscosity

4-Methylumbelliferyl-glycosides as fluorescence probes of sugar-binding sites on lectin molecules: Spectral properties and dependence of fluorescence on polarity and viscosity

The spectral properties of 4-methylumbelliferyl-glycosides (MeUmb-glycosides) were investigated in order to assess their usefulness as probes of the microenvironment of sugar binding sites on lectin molecules. It was shown that the abnormally high values for fluorescence polarization of free MeUmb-g...

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Veröffentlicht in:Archives of biochemistry and biophysics 1984-08, Vol.232 (2), p.640-653
Hauptverfasser: Decastel, Monique, Vincent, Michel, Matta, Khushi L., Frénoy, Jean-Pierre
Format: Artikel
Sprache:eng
Schlagworte:
Binding Sites
Carbohydrates - analysis
Electrochemistry
Fluorescence Polarization
Fluorescent Dyes - analysis
Glycosides - analysis
Hydrogen-Ion Concentration
Hymecromone - analogs & derivatives
Hymecromone - analysis
Lectins - analysis
Solvents
Spectrometry, Fluorescence
Temperature
Umbelliferones
Viscosity
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Zusammenfassung:The spectral properties of 4-methylumbelliferyl-glycosides (MeUmb-glycosides) were investigated in order to assess their usefulness as probes of the microenvironment of sugar binding sites on lectin molecules. It was shown that the abnormally high values for fluorescence polarization of free MeUmb-glycosides (from 0.07 to 0.251) were due neither to their molecular size nor to the blockade of their movement, but to the short lifetimes (
ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(84)90584-8