alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone α -factor. These cells accuratel...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1984-08, Vol.81 (15), p.4642-4646
Hauptverfasser: Brake, A.J, Merryweather, J.P, Coit, D.G, Heberlein, U.A, Masiarz, F.R, Mullenbach, G.T, Urdea, M.S, Valenzuela, P, Barr, P.J
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Amino acids
Base Sequence
Cell growth
Cloning, Molecular - methods
DNA
DNA, Recombinant
Epidermal Growth Factor - genetics
Epidermal Growth Factor - metabolism
Epithelial cells
Gels
Gene Expression Regulation
Genetic Vectors
Human growth
Mating Factor
Mutation
Open reading frames
Peptides - genetics
Plasmids
Protein Processing, Post-Translational
Saccharomyces cerevisiae - genetics
Secretion
Yeasts
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Zusammenfassung:Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone α -factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.81.15.4642