The effect of an arginyl to a cysteinyl replacement on the uncoating behaviour of a spherical plant virus

A mutant of cowpea chlorotic mottle virus is described which, when extracted in the absence of cysteine and diethyldithiocarbamate, is noninfective. The mutant virus apparently cannot be uncoated to initiate infection because phenol extracts of its RNA are infective. The mutant is more pH and salt s...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1971-09, Vol.45 (3), p.707-715
Hauptverfasser:	Bancroft, J.B, McLean, G.D, Rees, M.W, Short, Margaret N
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Arginine Autoanalysis Centrifugation, Density Gradient Chemical Phenomena Chemical Precipitation Chemistry Chromatography, Paper Cysteine Genetics, Microbial Hydrogen-Ion Concentration Mutation Peptides - analysis Phenols Plant Viruses - analysis Plant Viruses - pathogenicity Plants, Edible Plants, Medicinal RNA, Viral - isolation & purification RNA, Viral - pharmacology Sucrose Thiocarbamates Trypsin Viral Proteins - analysis
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Beschreibung
Zusammenfassung:	A mutant of cowpea chlorotic mottle virus is described which, when extracted in the absence of cysteine and diethyldithiocarbamate, is noninfective. The mutant virus apparently cannot be uncoated to initiate infection because phenol extracts of its RNA are infective. The mutant is more pH and salt stable than is the wild-type virus. The properties of the mutant are related to the replacement of an arginyl by a cysteinyl residue.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/0042-6822(71)90184-X