Microanalysis of Individual Mitochondrial Granules with Diameters Less Than 1000 Angstroms

Microanalysis of Individual Mitochondrial Granules with Diameters Less Than 1000 Angstroms

A scanning electron microscope was converted to an electron microprobe with high spatial resolution by the addition of a transmitted electron detector and a solid-state x-ray detector. Spectra obtained from mitochondrial granules of chondrocytes in situ confirm the suspected presence of calcium and...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1971-11, Vol.174 (4012), p.947-949
Hauptverfasser: Sutfin, Lloyd V., Holtrop, Marijke E., Ogilvie, Robert E.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bone and Bones - analysis
Bone and Bones - cytology
Calcium
Calcium - analysis
Cholecalciferols
Electron microscopes
Electron probes
Electrons
Methods
Mice
Microanalysis
Microscopy, Electron, Scanning
Mitochondria
Mitochondria - analysis
Phosphorus - analysis
Silicon - analysis
Spectrum Analysis
Strontium
Vacuum systems
Vitamin D
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Beschreibung
Zusammenfassung:A scanning electron microscope was converted to an electron microprobe with high spatial resolution by the addition of a transmitted electron detector and a solid-state x-ray detector. Spectra obtained from mitochondrial granules of chondrocytes in situ confirm the suspected presence of calcium and phosphorus. Contamination during analysis can lead to false indications of silicon in living tissue.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.174.4012.947