Identification and characterization of non-dividing cell populations in phase II cultures of human glial cells
It has been reported that cultures of normal cells in phase II contain a non-multiplying cell population, the size of which increases with passage number. In phase II cultures of normal human glial cells we have found two subpopulations of non-proliferating cells, one of which has a characteristic m...
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Veröffentlicht in: | Mechanisms of ageing and development 1984-01, Vol.26 (1), p.1-12 |
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Sprache: | eng |
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Zusammenfassung: | It has been reported that cultures of normal cells in phase II contain a non-multiplying cell population, the size of which increases with passage number. In phase II cultures of normal human glial cells we have found two subpopulations of non-proliferating cells, one of which has a characteristic morphology, and differs from the actively dividing cells in a number of respects: (1) they are larger although of various sizes and are well spread over a very large substratum area: (2) they contain a great number of granules showing acid phosphatase activity, being heavy metal positive and displaying the characteristic natural fluorescence of lipofuscin pigment; (3) they frequently contained a central somewhat irregular nucleus with various numbers of darkly straining nucleolar-like structures. Cytophotometric nuclear DNA measurements of the described “large” cell population show a decreased proportion of diploid cells as compared to their smaller sister cells. Moreover, with increasing passage number, the DNA values for large cells shift towards higher ploidy levels resulting in a scattered aneuploid pattern in the oldest passage. This “large” cell subpopulation consists of between 2% and 3% of all passages and become greatly decreased following subcultivation. The other subpopulation of non-dividing cells is generally morphologically similar to the dividers, increases in size with passage number and is the more important in the phase III phenomenon. |
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ISSN: | 0047-6374 1872-6216 |
DOI: | 10.1016/0047-6374(84)90160-X |