The effects of NaCl on the amino acid acceptor activity of rat liver tRNA

The effects of NaCl on the amino acid acceptor activity of rat liver tRNA

The effects of NaCl on the amino acid acceptor activity of rat liver tRNA was studied, and it was established that NaCl causes alterations of the acceptor activities for valine, lysine, leucine, and phenylalanine. Both the rate and the final level of the esterification of valine to tRNA was altered...

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Veröffentlicht in:Archives of biochemistry and biophysics 1971-07, Vol.145 (1), p.164-168
Hauptverfasser: Sein, K.T., Bećarević, A.
Format: Artikel
Sprache:eng
Schlagworte:
Acylation
Amino Acyl-tRNA Synthetases - antagonists & inhibitors
Amino Acyl-tRNA Synthetases - isolation & purification
Animals
Carbon Isotopes
Chromatography
Chromatography, DEAE-Cellulose
Kinetics
Leucine - metabolism
Liver - drug effects
Liver - enzymology
Liver - metabolism
Lysine - metabolism
Male
Methylation
Phenylalanine - metabolism
Phosphates - pharmacology
Rats
Rats, Inbred Strains
RNA, Transfer - metabolism
Serum Albumin
Silicon Dioxide
Sodium - pharmacology
Sodium Chloride - pharmacology
Tritium
Valine - metabolism
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Zusammenfassung:The effects of NaCl on the amino acid acceptor activity of rat liver tRNA was studied, and it was established that NaCl causes alterations of the acceptor activities for valine, lysine, leucine, and phenylalanine. Both the rate and the final level of the esterification of valine to tRNA was altered by NaCl. Formation of valyl-tRNA is more sensitive to NaH 2PO 4 than to the same molarity of NaCl. It was also found that valyl-tRNA synthesis is more sensitive to 0.3 m NaCl when a partially purified enzyme was used.
ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(71)90023-3