Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites

A cDNA library of P. falciparum was constructed. Using size-selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6-kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expre...

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Veröffentlicht in:	FEBS letters 1984-07, Vol.173 (1), p.108-112
Hauptverfasser:	Odink, Karel G., Lockyer, Michael J., Nicholls, Stephen C., Hillman, Yvonne, Freeman, Robert R., Holder, Anthony A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals antigens Antigens, Surface - genetics Biological and medical sciences Biotechnology cDNA cell surface Cloning, Molecular DNA - genetics Fundamental and applied biological sciences. Psychology Fusion polypeptide gene expression Gene Expression Regulation Genetic engineering Genetic technics genetics Human malaria merozoites Methods. Procedures. Technologies Molecular cloning Molecular Weight Nucleic acids, Protozoa Parasite surfaces Plasmodium falciparum Plasmodium falciparum - genetics Plasmodium falciparum - immunology Proteins, Protozoa Protozoa Surface antigen transformation
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Zusammenfassung:	A cDNA library of P. falciparum was constructed. Using size-selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6-kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expression vector. The expressed fusion protein was shown to contain epitopes of a 195-kDa protein which is the precursor to 3 major surface antigens of P. falciparum merozoites.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(84)81027-3