Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells

Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells

Injected frog eggs and oocytes provide a very sensitive assay system for the identification of messenger RNA and permit the study of translational control in living cells. The translation of each haemoglobin messenger RNA molecule once every 5–10 minutes for at least 24 hours makes it possible to re...

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Veröffentlicht in:Nature (London) 1971-09, Vol.233 (5316), p.177-182
Hauptverfasser: GURDON, J. B, LANE, C. D, WOODLAND, H. R, MARBAIX, G
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Anura
Cell-Free System
Chromatography, Ion Exchange
Female
Hemoglobins - biosynthesis
Histidine - metabolism
Humanities and Social Sciences
Injections
multidisciplinary
Ovary - cytology
Ovary - metabolism
Ovum - metabolism
Protein Biosynthesis
Proteins - analysis
Reticulocytes - metabolism
RNA, Messenger - pharmacology
Science
Science (multidisciplinary)
Tritium
Xenopus
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Beschreibung
Zusammenfassung:Injected frog eggs and oocytes provide a very sensitive assay system for the identification of messenger RNA and permit the study of translational control in living cells. The translation of each haemoglobin messenger RNA molecule once every 5–10 minutes for at least 24 hours makes it possible to recognize less than 1 ng of this messenger RNA.
ISSN:0028-0836
1476-4687
DOI:10.1038/233177a0