The preparation of freeze-thaw density gradients with homogeneous solute concentrations

A simple and effective method is described for preparing freeze-thaw density gradients which is free of the problems of uneven solute distribution. It consists of blending aqueous freeze-thaw gradients with hypertonic diluent. The routine application of this system for separations of murine lymphosa...

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Veröffentlicht in:	Journal of immunological methods 1984-07, Vol.71 (2), p.259-264
Hauptverfasser:	Cooper, A.J., Smallwood, J.A., Morgan, R.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell cultures. Hybridization. Fusion Cell Separation - methods Centrifugation, Density Gradient freeze-thaw density gradient Freezing Fundamental and applied biological sciences. Psychology General aspects Isotonic Solutions lymphocyte separation Lymphoma, Non-Hodgkin - pathology Mice Mice, Inbred CBA Molecular and cellular biology
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Beschreibung
Zusammenfassung:	A simple and effective method is described for preparing freeze-thaw density gradients which is free of the problems of uneven solute distribution. It consists of blending aqueous freeze-thaw gradients with hypertonic diluent. The routine application of this system for separations of murine lymphosarcoma cell populations is described.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(84)90072-3