Purification of Eimeria sporozoites by DE-52 anion exchange chromatography

Eimeria tenella, anion exchange column of DE-52 used to purify sporozoites from post-excystation mixture of oocysts, oocyst shells, sporocysts, sporocyst shells, and sporozoites, mean recovery was 94%, virtually all non-sporozoite material was removed, sporozoites were viable after purification and were at least as infectious as unpurified sporozoites, oocysts in crude preparation could be recovered from DE-52 cellulose by resuspending them in 20% (w/v) sodium chloride solution