New and versatile cloning vectors with kanamycin-resistance marker

New and versatile cloning vectors with kanamycin-resistance marker

Described here is a pair of small multi-copy kanamycin-resistance plasmids, containing the pUC lacZα- complementation peptide and the pUC18 and pUC19 multiple cloning site. These plasmids and their derivatives allow simple and rapid transfer of inserts from one replicon to another without the necess...

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Veröffentlicht in:Gene 1987, Vol.56 (2), p.309-312
1. Verfasser: Pridmore, Raymond David
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Biological and medical sciences
Biotechnology
cloning vectors
Cloning, Molecular
DNA, Bacterial - genetics
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genetic Vectors
Kanamycin Resistance - genetics
Methods. Procedures. Technologies
Molecular cloning
Molecular Sequence Data
polycloning sites
R Factors
Recombinant DNA
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Beschreibung
Zusammenfassung:Described here is a pair of small multi-copy kanamycin-resistance plasmids, containing the pUC lacZα- complementation peptide and the pUC18 and pUC19 multiple cloning site. These plasmids and their derivatives allow simple and rapid transfer of inserts from one replicon to another without the necessity of purifying the insert from vector.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(87)90149-1