A monoclonal antibody against a synthetic peptide reveals common structures among spectrins and α-actinin

A monoclonal antibody (Mab) against a synthetic peptide, SEDYGKDL, corresponding to one conserved sequence in the chicken α-fodrin repeats reacts in immunoblotting with avian α-spectrin and α-fodrin, both mammalian spectrins and with mammalian α-fodrin. This Mab also reacts with α-actinin in both ch...

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Veröffentlicht in:	FEBS letters 1987-11, Vol.224 (1), p.156-160
Hauptverfasser:	Närvänen, Outi, Närvänen, Ale, Wasenius, Veli-Matti, Partanen, Paul, Virtanen, Ismo
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinin - immunology alpha -actinin Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal - immunology Antibody Biological and medical sciences Carrier Proteins - immunology Chick Embryo Cross Reactions Cytoskeleton Epitopes - immunology Fundamental and applied biological sciences. Psychology Glycoproteins Humans Microfilament Proteins - immunology Peptides - chemical synthesis Peptides - immunology Phylogeny Proteins Sequence Homology, Nucleic Acid Spectrin Spectrin - immunology spectrins Synthetic peptide α-Actinin
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Beschreibung
Zusammenfassung:	A monoclonal antibody (Mab) against a synthetic peptide, SEDYGKDL, corresponding to one conserved sequence in the chicken α-fodrin repeats reacts in immunoblotting with avian α-spectrin and α-fodrin, both mammalian spectrins and with mammalian α-fodrin. This Mab also reacts with α-actinin in both chicken and human cells. Our results confirm the previously detected structural homology between spectrins and α-actinin and implicate their common evolutionary origin.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(87)80440-4