The isolation and properties of mitochondria from rat pancreas
A technique for the isolation of functional rat pancreatic mitochondria is described. The resultant mitochondrial preparations contained oligomycin-insensitive Mg 2+-ATPase activity and coupled respiration could only be demonstrated in the absence of Mg 2+ and in the presence of EDTA.
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| Veröffentlicht in: | Biochemical and biophysical research communications 1984-06, Vol.121 (2), p.545-551 |
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| container_end_page | 551 |
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| container_issue | 2 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 121 |
| creator | Wilson, J.S. Korsten, M.A. Lieber, C.S. |
| description | A technique for the isolation of functional rat pancreatic mitochondria is described. The resultant mitochondrial preparations contained oligomycin-insensitive Mg
2+-ATPase activity and coupled respiration could only be demonstrated in the absence of Mg
2+ and in the presence of EDTA. |
| doi_str_mv | 10.1016/0006-291X(84)90216-X |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1984-06, Vol.121 (2), p.545-551 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81113185 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphatases - metabolism adenosinetriphosphatase Animals Ca(2+) Mg(2+)-ATPase Cell Fractionation - methods Edetic Acid - pharmacology In Vitro Techniques Magnesium - pharmacology Male mitochondria Mitochondria - enzymology Mitochondria - metabolism Mitochondria - ultrastructure Oligomycins - pharmacology Oxygen Consumption - drug effects pancreas Pancreas - metabolism Pancreas - ultrastructure Rats Rats, Inbred Strains Succinate Dehydrogenase - metabolism |
| title | The isolation and properties of mitochondria from rat pancreas |
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