The isolation and properties of mitochondria from rat pancreas

The isolation and properties of mitochondria from rat pancreas

A technique for the isolation of functional rat pancreatic mitochondria is described. The resultant mitochondrial preparations contained oligomycin-insensitive Mg 2+-ATPase activity and coupled respiration could only be demonstrated in the absence of Mg 2+ and in the presence of EDTA.

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Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 1984-06, Vol.121 (2), p.545-551
Hauptverfasser: Wilson, J.S., Korsten, M.A., Lieber, C.S.
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphatases - metabolism
adenosinetriphosphatase
Animals
Ca(2+) Mg(2+)-ATPase
Cell Fractionation - methods
Edetic Acid - pharmacology
In Vitro Techniques
Magnesium - pharmacology
Male
mitochondria
Mitochondria - enzymology
Mitochondria - metabolism
Mitochondria - ultrastructure
Oligomycins - pharmacology
Oxygen Consumption - drug effects
pancreas
Pancreas - metabolism
Pancreas - ultrastructure
Rats
Rats, Inbred Strains
Succinate Dehydrogenase - metabolism
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Zusammenfassung:A technique for the isolation of functional rat pancreatic mitochondria is described. The resultant mitochondrial preparations contained oligomycin-insensitive Mg 2+-ATPase activity and coupled respiration could only be demonstrated in the absence of Mg 2+ and in the presence of EDTA.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(84)90216-X