High mobility group proteins HMG1 and HMG2 do not decrease the melting temperature of DNA

High mobility group proteins HMG1 and HMG2 do not decrease the melting temperature of DNA

High mobility group poteins 1 and 2 isolated in non-denaturing conditions cannot decrease the temperature of denaturation of DNA. When they are isolated or treated with tricloroacetic acid a hyperchromic peak below the melting temperature of free DNA appears in agreement with previous data ( Javaher...

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Veröffentlicht in:Biochemical and biophysical research communications 1984-05, Vol.120 (3), p.782-788
Hauptverfasser: Marekov, Lyuben N., Beltchev, Beltcho G., Pivec, Ladislav
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
Chromosomal Proteins, Non-Histone
DNA
High Mobility Group Proteins
Nucleic Acid Denaturation
Thermodynamics
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Zusammenfassung:High mobility group poteins 1 and 2 isolated in non-denaturing conditions cannot decrease the temperature of denaturation of DNA. When they are isolated or treated with tricloroacetic acid a hyperchromic peak below the melting temperature of free DNA appears in agreement with previous data ( Javaherian et al. (1979) Nucl. Acids Res. 6, 3569–3580). We show that this is due to light scattering of aggregated protein at submelting temperatures and not to melting of DNA.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(84)80175-8