An improved method for increasing the resolution and sensitivity of silver staining of nucleic acid bands in polyacrylamide gels

Staining of polyacrylamide gels with methylene blue prior to silver staining increases band resolution and sensitivity. This method permits resolution of multiple bands less than 1 mm apart, and is able to detect bands containing only 100 pg of RNA.

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Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 1984-04, Vol.138 (1), p.66-67
1. Verfasser:	Kolodny, Gerald M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Fundamental and applied biological sciences. Psychology General aspects, investigation methods Nucleic acids polyacrylamide gel resolution RNA RNA - analysis RNA, Bacterial - analysis RNA, Transfer - analysis Silver silver stain Staining and Labeling
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Beschreibung
Zusammenfassung:	Staining of polyacrylamide gels with methylene blue prior to silver staining increases band resolution and sensitivity. This method permits resolution of multiple bands less than 1 mm apart, and is able to detect bands containing only 100 pg of RNA.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(84)90769-3