A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the presence of dete...

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Veröffentlicht in:Analytical biochemistry 1984-04, Vol.138 (1), p.141-143
Hauptverfasser: Wessel, D., Flügge, U.I.
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cattle
Chemical Precipitation
Detergents
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Lipids
Osmolar Concentration
protein precipitation
Proteins
Proteins - analysis
Rabbits
Rats
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Solutions
TX-100
β-mercaptoethanol
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Zusammenfassung:A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the presence of detergents, lipids, salt, buffers, and β-mercaptoethanol.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(84)90782-6