Purification of Quadrol for microsequencing in the spinning-cup sequenator

Treatment of Quadrol buffer with isothiocyanatophenylthiocarbamylaminoethylaminopropyl glass (DITC glass) substantially reduces the impurities observed when this buffer is used with the spinning cup sequenator. Use of DITC glass-treated Quadrol buffer permits identification of PTH-amino acids from p...

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Veröffentlicht in:	Analytical biochemistry 1984-01, Vol.138 (1), p.30-33
Hauptverfasser:	Begg, G.S., Leslie, Barbara H., Morgan, F.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acids Analytical, structural and metabolic biochemistry Autoanalysis - instrumentation Biological and medical sciences Buffers - isolation & purification Chromatography - methods Ethylenediamines - isolation & purification Fundamental and applied biological sciences. Psychology General aspects, investigation methods Microchemistry microsequencing phenylthiohydantoin Proteins quadrol spinning cup sequenator
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Beschreibung
Zusammenfassung:	Treatment of Quadrol buffer with isothiocyanatophenylthiocarbamylaminoethylaminopropyl glass (DITC glass) substantially reduces the impurities observed when this buffer is used with the spinning cup sequenator. Use of DITC glass-treated Quadrol buffer permits identification of PTH-amino acids from protein degradations down to 100 pmol.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(84)90764-4