Column purification of adenovirus cores

A simple and efficient technique for purifying adenoviral chromatin (nucleoprotein cores) with Sephacryl S-1000 is described. This method is significantly faster than previous methods and gives a higher degree of purity with an increased recovery of the nucleoprotein. In addition, the structural int...

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Veröffentlicht in:	Analytical biochemistry 1987-07, Vol.164 (1), p.267-270
Hauptverfasser:	Cupo, J.F., Rezanka, L.J., Harpst, J.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylic Resins adenovirus Adenoviruses, Human - analysis Biological and medical sciences chromatography Chromatography, Gel DNA-protein interaction electron microscopy DNA-protein Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Microbiology Microscopy, Electron Molecular Weight Morphology, structure, chemical composition, physicochemical properties nucleic acid structure nucleic acids ultracentrifugation Viral Core Proteins - isolation & purification Virology
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Beschreibung
Zusammenfassung:	A simple and efficient technique for purifying adenoviral chromatin (nucleoprotein cores) with Sephacryl S-1000 is described. This method is significantly faster than previous methods and gives a higher degree of purity with an increased recovery of the nucleoprotein. In addition, the structural integrity of the cores is maintained.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(87)90396-4