Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers
A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped...
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| Veröffentlicht in: | Analytical biochemistry 1987-07, Vol.164 (1), p.39-43 |
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| container_end_page | 43 |
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| container_issue | 1 |
| container_start_page | 39 |
| container_title | Analytical biochemistry |
| container_volume | 164 |
| creator | Bodhe, A.M. Vartak, H.G. Rele, M.V. Dhamankar, V.S. |
| description | A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped in locating the proteins. The located desired protein could subsequently be purified by extraction. |
| doi_str_mv | 10.1016/0003-2697(87)90364-2 |
| format | Article |
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Psychology ; gel electrophoresis ; General aspects, investigation methods ; peptides ; Plant Proteins - isolation & purification ; Plants - analysis ; polyacrylamide gel electrophoresis ; protein purification ; proteins ; Serum Albumin, Bovine - isolation & purification ; Streptomyces - enzymology ; Subtilisins - antagonists & inhibitors</subject><ispartof>Analytical biochemistry, 1987-07, Vol.164 (1), p.39-43</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-67981027b57e6e828754472aabbb81af2d443ce48c660fcbd89a0e8147e04fa83</citedby><cites>FETCH-LOGICAL-c417t-67981027b57e6e828754472aabbb81af2d443ce48c660fcbd89a0e8147e04fa83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(87)90364-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7658780$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3314580$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bodhe, A.M.</creatorcontrib><creatorcontrib>Vartak, H.G.</creatorcontrib><creatorcontrib>Rele, M.V.</creatorcontrib><creatorcontrib>Dhamankar, V.S.</creatorcontrib><title>Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped in locating the proteins. The located desired protein could subsequently be purified by extraction.</description><subject>Aldose-Ketose Isomerases</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Epimerases - isolation & purification</subject><subject>casein</subject><subject>Dialysis</subject><subject>dialyzable fluorescent markers</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>enzymes</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fluorescamine</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gel electrophoresis</subject><subject>General aspects, investigation methods</subject><subject>peptides</subject><subject>Plant Proteins - isolation & purification</subject><subject>Plants - analysis</subject><subject>polyacrylamide gel electrophoresis</subject><subject>protein purification</subject><subject>proteins</subject><subject>Serum Albumin, Bovine - isolation & purification</subject><subject>Streptomyces - enzymology</subject><subject>Subtilisins - antagonists & inhibitors</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EKtvCG4DkA0LlkDJOvLZzqYQqoEgr9QJn4zjjyuDEi50gpU-PtxvtkZ48nvnm18w_hLxhcMWAiY8A0FS1aOWlkh9aaASv6mdkw6AVFTTQPiebE_KSnOf8C4AxvhVn5KxpSqBgQ37uojWTjyM1Y0_3c_LOr4noKI4Py4CZlt8-hsXYtAQz-B7pPYZM5-zHe9p7E5YH0wWkLswxYbY4TnQw6Tem_Iq8cCZkfL2-F-THl8_fb26r3d3XbzefdpXlTE6VkK1iUMtuK1GgqpXcci5rY7quU8y4uue8sciVFQKc7XrVGkDFuETgzqjmgrw_6u5T_DNjnvTgyyAhmBHjnHVRb4sdT4OMK2ibti4gP4I2xZwTOr1Pvmy1aAb6cAF9sFcf7NVK6scL6EPb21V_7gbsT02r5aX-bq2bbE1wyYzW5xMmxVbJR-z6iBWj8a_HpLP1OFrsfUI76T76_8_xD-ftotA</recordid><startdate>19870701</startdate><enddate>19870701</enddate><creator>Bodhe, A.M.</creator><creator>Vartak, H.G.</creator><creator>Rele, M.V.</creator><creator>Dhamankar, V.S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19870701</creationdate><title>Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers</title><author>Bodhe, A.M. ; Vartak, H.G. ; Rele, M.V. ; Dhamankar, V.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-67981027b57e6e828754472aabbb81af2d443ce48c660fcbd89a0e8147e04fa83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Aldose-Ketose Isomerases</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Epimerases - isolation & purification</topic><topic>casein</topic><topic>Dialysis</topic><topic>dialyzable fluorescent markers</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>enzymes</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fluorescamine</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gel electrophoresis</topic><topic>General aspects, investigation methods</topic><topic>peptides</topic><topic>Plant Proteins - isolation & purification</topic><topic>Plants - analysis</topic><topic>polyacrylamide gel electrophoresis</topic><topic>protein purification</topic><topic>proteins</topic><topic>Serum Albumin, Bovine - isolation & purification</topic><topic>Streptomyces - enzymology</topic><topic>Subtilisins - antagonists & inhibitors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bodhe, A.M.</creatorcontrib><creatorcontrib>Vartak, H.G.</creatorcontrib><creatorcontrib>Rele, M.V.</creatorcontrib><creatorcontrib>Dhamankar, V.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bodhe, A.M.</au><au>Vartak, H.G.</au><au>Rele, M.V.</au><au>Dhamankar, V.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1987-07-01</date><risdate>1987</risdate><volume>164</volume><issue>1</issue><spage>39</spage><epage>43</epage><pages>39-43</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. 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| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1987-07, Vol.164 (1), p.39-43 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Aldose-Ketose Isomerases Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrate Epimerases - isolation & purification casein Dialysis dialyzable fluorescent markers Electrophoresis, Polyacrylamide Gel enzymes Enzymes and enzyme inhibitors Fluorescamine Fluorescence Fluorescent Dyes Fundamental and applied biological sciences. Psychology gel electrophoresis General aspects, investigation methods peptides Plant Proteins - isolation & purification Plants - analysis polyacrylamide gel electrophoresis protein purification proteins Serum Albumin, Bovine - isolation & purification Streptomyces - enzymology Subtilisins - antagonists & inhibitors |
| title | Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers |
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