Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers

A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped...

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Veröffentlicht in:	Analytical biochemistry 1987-07, Vol.164 (1), p.39-43
Hauptverfasser:	Bodhe, A.M., Vartak, H.G., Rele, M.V., Dhamankar, V.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldose-Ketose Isomerases Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrate Epimerases - isolation & purification casein Dialysis dialyzable fluorescent markers Electrophoresis, Polyacrylamide Gel enzymes Enzymes and enzyme inhibitors Fluorescamine Fluorescence Fluorescent Dyes Fundamental and applied biological sciences. Psychology gel electrophoresis General aspects, investigation methods peptides Plant Proteins - isolation & purification Plants - analysis polyacrylamide gel electrophoresis protein purification proteins Serum Albumin, Bovine - isolation & purification Streptomyces - enzymology Subtilisins - antagonists & inhibitors
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Beschreibung
Zusammenfassung:	A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped in locating the proteins. The located desired protein could subsequently be purified by extraction.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(87)90364-2