Theiler's Virus-specified Polypeptides Made in BHK-21 Cells

Department of Neurology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, Illinois 60611, U.S.A. and 1 Animal Virus Research Institute, Pirbright, Woking, Surrey, U.K. This study was initiated to determine whether the Theiler's murine encephalomyelitis viruses (TMEV) us...

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Veröffentlicht in:	Journal of general virology 1984-06, Vol.65 (6), p.1095-1100
Hauptverfasser:	Lipton, Howard L, Rozhon, Edward J, Black, Donald
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Line Chromosome Mapping Cricetinae Enterovirus - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Genes, Viral - drug effects Kidney - metabolism Kidney - microbiology Maus Elberfeld virus - drug effects Maus Elberfeld virus - genetics Maus Elberfeld virus - metabolism Microbiology Pactamycin - pharmacology Peptide Biosynthesis Peptides - genetics Protein Processing, Post-Translational - drug effects Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Viral Proteins - biosynthesis Viral Proteins - genetics Virology Virus Cultivation
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Zusammenfassung:	Department of Neurology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, Illinois 60611, U.S.A. and 1 Animal Virus Research Institute, Pirbright, Woking, Surrey, U.K. This study was initiated to determine whether the Theiler's murine encephalomyelitis viruses (TMEV) use the same strategy of gene expression as other picornaviruses. The precursor-product relationships apparent from pulse-chase experiments indicated that the virus-specified polypeptides of the GDVII strain are generated by post-translational cleavages. Pactamycin mapping experiments also showed a gene order for GDVII virus similar to that derived for other picornaviruses by this technique. Finally, six other TMEV induced species of polypeptides similar to those of GDVII virus, but there were minor differences in some of their electrophoretic mobilities. Keywords: Theiler's virus, enterovirus, pactamycin, neurovirulence Received 7 November 1983; accepted 23 February 1984.
ISSN:	0022-1317 1465-2099
DOI:	10.1099/0022-1317-65-6-1095