The preparation and properties of novel reversible polymer-protein conjugates 2-ω-Methoxypolyethylene (5000) glycoxymethylene-3-methylmaleyl conjugates of plasminogen activators
The preparation of a reagent capable of reversibly attaching polyethylene glycol to proteins and the use of this material in modifying the plasminogen activators urokinase- and tissue-type plasminogen activator are described. The characterisation and the reversible nature of these protein-polymer co...
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| Veröffentlicht in: | FEBS letters 1987-11, Vol.223 (2), p.361-365 |
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| container_title | FEBS letters |
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| creator | Garman, Andrew J. Barret Kalindjian, S. |
| description | The preparation of a reagent capable of reversibly attaching polyethylene glycol to proteins and the use of this material in modifying the plasminogen activators urokinase- and tissue-type plasminogen activator are described. The characterisation and the reversible nature of these protein-polymer conjugates are discussed, and some of the in vitro properties of these modified enzymes are explored. |
| doi_str_mv | 10.1016/0014-5793(87)80319-8 |
| format | Article |
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Technologies</subject><subject>Plasminogen activator</subject><subject>Polyethylene Glycols</subject><subject>Polymers</subject><subject>Tissue Plasminogen Activator - metabolism</subject><subject>Tissue Plasminogen Activator - pharmacokinetics</subject><subject>Urokinase</subject><subject>Urokinase-Type Plasminogen Activator - metabolism</subject><subject>Urokinase-Type Plasminogen Activator - pharmacokinetics</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAQxy0EKsvCG4CUA0LtwWDHztq-IEHVhUpFXMrZcpzJ1pUTL3Z2IY_A6_AivBJOs6w4FU6ej9_MeOaP0HNKXlNCV28IoRxXQrFTKc4kYVRh-QAtqBQMM76SD9HiiDxGT1K6JdmXVJ2gE0apqKRcoJ_XN1BsI2xNNIMLfWH6JvthC3FwkIrQFn3Ygy8i7CEmV_uMBz92EHHGBnB9YUN_u9uYIeMl_vUDf4LhJnwfJyxbo4ceitMqDz8rNn60OdX9iWOGZ7szHkb_d6s8eetN6lwfNpD_ZQe3N0OI6Sl61Bqf4NnhXaIv64vr84_46vOHy_N3V9hyTiWmvG4b4HWzkqvaSmGVqgWpGC1VC4Y3hFtlJGXMiqaCtlalNU2tFGtKCrS2bIlezX3znl93kAbduWTBe9ND2CUtKRFUcP5PkFYlJaWaQD6DNoaUIrR6G11n4qgp0ZOmehJMT4JpKfSdplrmsheH_ru6g-ZYdBAx518e8iZZ49toeuvSEROC0WnPJVrP2DeXb_1fo_X64n05Jaa4FHfRad7buRHk6-8dRJ2sg95C4yLYQTfB3b_QbwPe1pw</recordid><startdate>19871102</startdate><enddate>19871102</enddate><creator>Garman, Andrew J.</creator><creator>Barret Kalindjian, S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19871102</creationdate><title>The preparation and properties of novel reversible polymer-protein conjugates 2-ω-Methoxypolyethylene (5000) glycoxymethylene-3-methylmaleyl conjugates of plasminogen activators</title><author>Garman, Andrew J. ; Barret Kalindjian, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4418-14bfde4bd686bc87c99b7053129fea4d04c9a8133c7d5efb92cadb993d21e1bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chemical modification</topic><topic>Fundamental and applied biological sciences. 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The characterisation and the reversible nature of these protein-polymer conjugates are discussed, and some of the in vitro properties of these modified enzymes are explored.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3117588</pmid><doi>10.1016/0014-5793(87)80319-8</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1987-11, Vol.223 (2), p.361-365 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81071744 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present); Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Biotechnology Chemical modification Fundamental and applied biological sciences. Psychology Guinea Pigs Immobilization of enzymes and other molecules Immobilization techniques Maleic Anhydrides Methods. Procedures. Technologies Plasminogen activator Polyethylene Glycols Polymers Tissue Plasminogen Activator - metabolism Tissue Plasminogen Activator - pharmacokinetics Urokinase Urokinase-Type Plasminogen Activator - metabolism Urokinase-Type Plasminogen Activator - pharmacokinetics |
| title | The preparation and properties of novel reversible polymer-protein conjugates 2-ω-Methoxypolyethylene (5000) glycoxymethylene-3-methylmaleyl conjugates of plasminogen activators |
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