A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS

A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Brain research 1984-04, Vol.298 (2), p.235-241
Hauptverfasser:	Gibson, A.R., Hansma, D.I., Houk, J.C., Robinson, F.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Axonal Transport Benzidines - metabolism Biological and medical sciences Brain - anatomy & histology Cats Cerebellar Cortex - anatomy & histology Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Histological Techniques Horseradish Peroxidase - metabolism lectin Lectins - metabolism Nerve Fibers - ultrastructure Olivary Nucleus - anatomy & histology Peroxidases - metabolism Pons - anatomy & histology Rats Red Nucleus - anatomy & histology Retina - anatomy & histology Spinal Cord - anatomy & histology Staining and Labeling TMB Vertebrates: nervous system and sense organs Visual Pathways - anatomy & histology WGA-HRP wheat germ agglutinin Wheat Germ Agglutinins
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.
ISSN:	0006-8993 1872-6240
DOI:	10.1016/0006-8993(84)91423-9