Visualization of pronuclei in living bovine zygotes

Bovine embryos were surgically collected from the oviducts of superovulated crossbred heifers 48 h postonset of estrus. The one-celled ova were treated with 4'-6'-diamidino-2-phenylindole (DAPI) and observed under ultraviolet light by fluorescence microscopy. Both male and female pronuclei...

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Veröffentlicht in:	Biology of reproduction 1984-04, Vol.30 (3), p.687-691
Hauptverfasser:	Minhas, B.S, Capehart, J.S, Bowen, M.J, Womack, J.E, Harms, P.G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cattle Cell Nucleus - ultrastructure Early stages. Segmentation. Gastrulation. Neurulation Embryology: invertebrates and vertebrates. Teratology Female Fertilization Fundamental and applied biological sciences. Psychology Microscopy, Fluorescence Pregnancy Superovulation Zygote - ultrastructure
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Beschreibung
Zusammenfassung:	Bovine embryos were surgically collected from the oviducts of superovulated crossbred heifers 48 h postonset of estrus. The one-celled ova were treated with 4'-6'-diamidino-2-phenylindole (DAPI) and observed under ultraviolet light by fluorescence microscopy. Both male and female pronuclei were visualized, identified and subjected to micromanipulation.
ISSN:	0006-3363 1529-7268
DOI:	10.1095/biolreprod30.3.687