Generation of excited species catalyzed by horseradish peroxidase or hemin in the presence of reduced glutathione and H2O2

Horseradish peroxidase (HRP) (EC 1.11.1.7) catalyzes the oxidation of reduced glutathione. This reaction is accompanied by light emission, which is attributed to the generation of singlet oxygen. The chemiluminescence is directly related to thiyl radical formation, as deduced from the correlation be...

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Veröffentlicht in:	Free radical biology & medicine 1987, Vol.3 (2), p.107-110
Hauptverfasser:	MEDEIROS, M. H. G, WEFERS, H, SIES, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Fundamental and applied biological sciences. Psychology Glutathione - analogs & derivatives Glutathione - metabolism Glutathione Disulfide Heme - metabolism Horseradish Peroxidase - metabolism Hydrogen Peroxide - metabolism Kinetics Luminescent Measurements Miscellaneous Molecular biophysics Oxidation-Reduction Peroxidases - metabolism Radiation-biomolecule interaction Spectrophotometry - methods
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Zusammenfassung:	Horseradish peroxidase (HRP) (EC 1.11.1.7) catalyzes the oxidation of reduced glutathione. This reaction is accompanied by light emission, which is attributed to the generation of singlet oxygen. The chemiluminescence is directly related to thiyl radical formation, as deduced from the correlation between the time course of HRP-compound II formation and light emission in the presence of different amounts of H2O2. Superoxide dismutase has an inhibitory effect on the chemiluminescence without affecting the HRP-compound II formation. This indicates the direct involvement of superoxide radicals in the production of photoemissive species. Replacement of HRP by hemin is also accompanied by chemiluminescence.
ISSN:	0891-5849 1873-4596
DOI:	10.1016/s0891-5849(87)80004-7