N-glycansulfated fibronectin: One of the several sulfated glycoproteins synthesized by endothelial cells in culture

N-glycanase, an endoglycosidase that cleaves the bond between asparagine and glucosamine, releases oligosaccharides with various degree of sulfation from endothelial cell fibronetin. As shown by analysis by polyacrylamide gel electrophoresis of culture medium conditioned by cells exposed to [ 35S]su...

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Veröffentlicht in:Biochemical and biophysical research communications 1987-09, Vol.147 (3), p.920-926
Hauptverfasser: Colburn, P., Buonassisi, V., Dietrich, C.P., Nader, H.B.
Format: Artikel
Sprache:eng
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Zusammenfassung:N-glycanase, an endoglycosidase that cleaves the bond between asparagine and glucosamine, releases oligosaccharides with various degree of sulfation from endothelial cell fibronetin. As shown by analysis by polyacrylamide gel electrophoresis of culture medium conditioned by cells exposed to [ 35S]sulfate, endothelial cell fibronectin is one of a number of glycoproteins bearing sulfated oligosaccharides, synthesized by this cell type.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(87)80158-4