Transformation of yeast spheroplasts without cell fusion

The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 10 7 and 5 × 10 6 transformants/μg, respe...

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Veröffentlicht in:	Analytical biochemistry 1987-06, Vol.163 (2), p.391-397
Hauptverfasser:	Burgers, Peter M.J., Percival, Kimberly J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences Cell Fusion DNA, Fungal - genetics DNA, Single-Stranded - genetics Exact sciences and technology Other techniques and industries Plasmids protoplasts Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Spheroplasts transformation Transformation, Genetic
Online-Zugang:	Volltext
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