Transformation of yeast spheroplasts without cell fusion

Transformation of yeast spheroplasts without cell fusion

The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 10 7 and 5 × 10 6 transformants/μg, respe...

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Veröffentlicht in:Analytical biochemistry 1987-06, Vol.163 (2), p.391-397
Hauptverfasser: Burgers, Peter M.J., Percival, Kimberly J.
Format: Artikel
Sprache:eng
Schlagworte:
Applied sciences
Cell Fusion
DNA, Fungal - genetics
DNA, Single-Stranded - genetics
Exact sciences and technology
Other techniques and industries
Plasmids
protoplasts
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Spheroplasts
transformation
Transformation, Genetic
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Beschreibung
Zusammenfassung:The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 10 7 and 5 × 10 6 transformants/μg, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(87)90240-5