[11] Optimal pH conditions for ion exchangers on macroporous supports

Two techniques are available for determining the most suitable pH conditions for performing ion-exchange chromatography with macroporous media: the electrophoretic titration curve approach and the retention mapping method. The ready determination of this parameter should provide optimal conditions f...

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Veröffentlicht in:Methods in Enzymology 1984, Vol.104, p.223-233
1. Verfasser: Richey, James S.
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description Two techniques are available for determining the most suitable pH conditions for performing ion-exchange chromatography with macroporous media: the electrophoretic titration curve approach and the retention mapping method. The ready determination of this parameter should provide optimal conditions for truly high-performance ion-exchange chromatography of biologically active macromolecules. Both methods are known to be effective only with macroporous media.
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subjects Animals
Carbonic Anhydrases - isolation & purification
Chromatography, Ion Exchange - methods
electrophoresis
Electrophoresis, Polyacrylamide Gel
Enzymes - isolation & purification
Hydrogen-Ion Concentration
ion-exchange chromatography
Isoenzymes
L-Lactate Dehydrogenase - isolation & purification
Proteins - isolation & purification
Resins, Synthetic
titration
title [11] Optimal pH conditions for ion exchangers on macroporous supports
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