[11] Optimal pH conditions for ion exchangers on macroporous supports

Two techniques are available for determining the most suitable pH conditions for performing ion-exchange chromatography with macroporous media: the electrophoretic titration curve approach and the retention mapping method. The ready determination of this parameter should provide optimal conditions f...

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Veröffentlicht in:	Methods in Enzymology 1984, Vol.104, p.223-233
1. Verfasser:	Richey, James S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Carbonic Anhydrases - isolation & purification Chromatography, Ion Exchange - methods electrophoresis Electrophoresis, Polyacrylamide Gel Enzymes - isolation & purification Hydrogen-Ion Concentration ion-exchange chromatography Isoenzymes L-Lactate Dehydrogenase - isolation & purification Proteins - isolation & purification Resins, Synthetic titration
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Zusammenfassung:	Two techniques are available for determining the most suitable pH conditions for performing ion-exchange chromatography with macroporous media: the electrophoretic titration curve approach and the retention mapping method. The ready determination of this parameter should provide optimal conditions for truly high-performance ion-exchange chromatography of biologically active macromolecules. Both methods are known to be effective only with macroporous media.
ISSN:	0076-6879 1557-7988
DOI:	10.1016/S0076-6879(84)04092-1