Crystallization and preliminary X-ray study of pig lens aldose reductase

Crystals of pig lens aldose reductase have been grown from polyethylene glycol solutions at pH 6.2 and analysed by X-ray diffraction. Two crystal forms were obtained. The first belongs to space group P1 with unit cell dimensions a = 81.3 A ̊ , b = 85.9 A ̊ , c = 56.6 A ̊ , α = 102.3 °, β = 103.3 °, γ = 79.0 ° , with four molecules in the unit cell related by a 222 non-crystallographic symmetry. The second crystal form is hexagonal. The space group is P6(2)22 with a = b = 101 A ̊ , c = 257 A ̊ and two molecules in the asymmetric unit. Both forms are suitable for X-ray structure analysis to better than 3 Å resolution.