Dialysis of bovine semen and its effect on fresh and freeze-thawed spermatozoa

The effect of the removal of the low-molecular-weight fraction (LMWF, 0.05) in sperm post-thaw motility were found after evaluation of the number of cells that passed through the Sephadex filter and all the dialyzed values obtained were significantly ( P < 0.05) superior to the results obtained with no dialysis. These results indicated that percentage motility of post-thawed spermatozoa was improved by the removal of the LMWF from seminal plasma and egg yolk by dialysis during the cooling process. Also, the substances removed that are deleterious have a MWCO smaller than 1,000 Da. This fraction may include sugars, free amino acids, free ions, and metabolites.