High efficiency capillary column-gas chromatography mass spectrometry : Analysis of the lipoxygenase pathway in eukaryot cells

Lipoxygenases are ubiquitous enzymes able to oxygenate polyunsaturated fatty acids. This metabolic pathway leads to hydroperoxides, hydroxyepoxyene compounds and leukotrienes. Using high performance gas chromatography prior to mass spectrometry, we studied the activity of the lipoxygenases from mous...

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Veröffentlicht in:	Prostaglandins leukotrienes and medicine 1984, Vol.13 (1), p.9-13
Hauptverfasser:	Rabinovitch-Chable, H., Durand, J., Aldigier, J.C., Chebroux, P., Gualde, N., Beneytout, J.L., Rigaud, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Arachidonic Acid Arachidonic Acids - metabolism Biological and medical sciences Chromatography, High Pressure Liquid Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry - methods Lipid Peroxides - metabolism Lipoxygenase - metabolism Macrophages - enzymology Mice Transferases
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Beschreibung
Zusammenfassung:	Lipoxygenases are ubiquitous enzymes able to oxygenate polyunsaturated fatty acids. This metabolic pathway leads to hydroperoxides, hydroxyepoxyene compounds and leukotrienes. Using high performance gas chromatography prior to mass spectrometry, we studied the activity of the lipoxygenases from mouse peritoneal macrophages. Further studies on mechanism of biosynthesis of hydroxyepoxyene compounds were successfully carried out using 18O 2 labelled precursors.
ISSN:	0262-1746
DOI:	10.1016/0262-1746(84)90096-9