A simple method for immune analysis of proteins following sodium dodecyl sulphate polyacrylamide gel electrophoresis

Immune analysis of polypeptides separated by SDS-PAGE provides a technique useful in the characterisation of complex mixtures. We describe a simple procedure involving immune analysis of fractionated PAGE gels. Polypeptides of purified herpesvirus simplex, HSV ‘excreted antigen’ and cell culture ‘co...

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Veröffentlicht in:	Journal of immunological methods 1984-03, Vol.67 (2), p.347-351
Hauptverfasser:	Cleator, G.M., Klapper, P.E., Cropper, L.M., Coupes, D., Longson, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Antigens, Viral - immunology Biological and medical sciences Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology gel electrophoresis herpesvirus antigens immune analysis of proteins Mice Mice, Inbred BALB C Microbiology Radioimmunoassay - methods Simplexvirus - immunology Sodium Dodecyl Sulfate Techniques used in virology Viral Envelope Proteins Viral Proteins - immunology Virology
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Zusammenfassung:	Immune analysis of polypeptides separated by SDS-PAGE provides a technique useful in the characterisation of complex mixtures. We describe a simple procedure involving immune analysis of fractionated PAGE gels. Polypeptides of purified herpesvirus simplex, HSV ‘excreted antigen’ and cell culture ‘control’ preparations were separated by SDS-PAGE. Following electrophoresis, protein was eluted from gels, adsorbed to plastic and analysed using an RIA technique. When using a mouse anti-HSV1 serum, 7 peaks of immune reactivity were observed with pure virus and 6 with HSV ‘excreted antigen’. Only 1 peak of reactivity was observed with a ‘control’ antigen.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(84)90474-5