A rapid, simplified procedure for simultaneous assay of norepinephrine, dopamine, and 5-hydroxytryptamine from discrete brain areas

A rapid, simplified procedure for simultaneous assay of norepinephrine, dopamine, and 5-hydroxytryptamine from discrete brain areas

Brain, NE, DA, and 5-HT can be assayed simultaneously from discrete samples by this method, which is based upon the alumina method described by Anton and Sayre, utilizing acid extraction of tissues and a single solvent step. Oxidation and determination of both NE and DA take place in a single sample...

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Veröffentlicht in:Analytical biochemistry 1971-02, Vol.39 (2), p.356-372
Hauptverfasser: Shellenberger, M.Kent, Gordon, J.H.
Format: Artikel
Sprache:eng
Schlagworte:
Aluminum
Animals
Brain Chemistry
Catecholamines - isolation & purification
Cerebellum - analysis
Cerebral Cortex - analysis
Dopamine - analysis
Hydrogen-Ion Concentration
Indicators and Reagents
Medulla Oblongata - analysis
Methods
Microchemistry
Norepinephrine - analysis
Oxidation-Reduction
Oxides
Pons - analysis
Rats
Serotonin - analysis
Serotonin - isolation & purification
Solvents
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Zusammenfassung:Brain, NE, DA, and 5-HT can be assayed simultaneously from discrete samples by this method, which is based upon the alumina method described by Anton and Sayre, utilizing acid extraction of tissues and a single solvent step. Oxidation and determination of both NE and DA take place in a single sample and may be completed within 1–1.5 hr. Basic extractions of 5-HT and the development of a tissue blank procedure permit the accurate application of the ninhydrin reaction to the determination of 5-HT, resulting in greatly increased sensitivity.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(71)90426-X