The spatial organization of nucleolar DNA in phytohemagglutinin-stimulated lymphocytes of the guinea pig
The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstructio...
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| Veröffentlicht in: | Cell and tissue research 1984, Vol.235 (1), p.65-70 |
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| container_title | Cell and tissue research |
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| creator | ANTEUNIS, A POUCHELET, M GANSMULLER, A ROBINEAUX, R |
| description | The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstruction of serial sections. The extended DNA filaments situated inside the fibrillar area originate from a large aggregation of heterochromatin, which is closely associated with the nucleolus, and from the perinucleolar shell of condensed chromatin. It is suggested that these two distinct regions of chromatin might be associated with different functions. |
| doi_str_mv | 10.1007/BF00213724 |
| format | Article |
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The extended DNA filaments situated inside the fibrillar area originate from a large aggregation of heterochromatin, which is closely associated with the nucleolus, and from the perinucleolar shell of condensed chromatin. It is suggested that these two distinct regions of chromatin might be associated with different functions.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/BF00213724</identifier><identifier>PMID: 6697385</identifier><identifier>CODEN: CTSRCS</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; Cell Nucleolus - ultrastructure ; Cell nucleus ; Cell structures and functions ; Chromatin - ultrastructure ; DNA - analysis ; Fundamental and applied biological sciences. 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The extended DNA filaments situated inside the fibrillar area originate from a large aggregation of heterochromatin, which is closely associated with the nucleolus, and from the perinucleolar shell of condensed chromatin. It is suggested that these two distinct regions of chromatin might be associated with different functions.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleolus - ultrastructure</subject><subject>Cell nucleus</subject><subject>Cell structures and functions</subject><subject>Chromatin - ultrastructure</subject><subject>DNA - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - ultrastructure</subject><subject>Molecular and cellular biology</subject><subject>Nucleolus Organizer Region - ultrastructure</subject><subject>Phytohemagglutinins - pharmacology</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EKtvChTuSD4gDUsrYju3k2BYKlapyKRK3aNbrJEaOHWLnsPz6ettVOXKZz2fekV5C3jE4ZwD68-U1AGdC8_oF2bBa8Aoa3bwkGxDAK63Ur9fkNKXfAKxWqj0hJyVq0cgNGe9HS9OM2aGncRkwuL-liYHGnobVeBs9LvTL3QV1gc7jPsfRTjgMfs0uuFCl7KbVY7Y76vfTPEazzzYdrnNRHlYXLNLZDW_Iqx59sm-P-Yz8vP56f_W9uv3x7ebq4rYyoua50tBaxdVWyl5DLcpQGy5EKVhjARSry1xL26IxKGGL3OpdL0UvAetGgTgjH5905yX-WW3K3eSSsd5jsHFNXQOt1MWd_4JMtLLm7QH89ASaJaa02L6bFzfhsu8YdAf_u3_-F_j9UXXdTnb3jB4NL_sPxz0mg75fMBiXnrHHl6oVD3DUjNI</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>ANTEUNIS, A</creator><creator>POUCHELET, M</creator><creator>GANSMULLER, A</creator><creator>ROBINEAUX, R</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1984</creationdate><title>The spatial organization of nucleolar DNA in phytohemagglutinin-stimulated lymphocytes of the guinea pig</title><author>ANTEUNIS, A ; POUCHELET, M ; GANSMULLER, A ; ROBINEAUX, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-709e626b55f7043c347c2333c318e00614f7075e9acca50ba2e7df53f50a48603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleolus - ultrastructure</topic><topic>Cell nucleus</topic><topic>Cell structures and functions</topic><topic>Chromatin - ultrastructure</topic><topic>DNA - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - ultrastructure</topic><topic>Molecular and cellular biology</topic><topic>Nucleolus Organizer Region - ultrastructure</topic><topic>Phytohemagglutinins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ANTEUNIS, A</creatorcontrib><creatorcontrib>POUCHELET, M</creatorcontrib><creatorcontrib>GANSMULLER, A</creatorcontrib><creatorcontrib>ROBINEAUX, R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ANTEUNIS, A</au><au>POUCHELET, M</au><au>GANSMULLER, A</au><au>ROBINEAUX, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The spatial organization of nucleolar DNA in phytohemagglutinin-stimulated lymphocytes of the guinea pig</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>1984</date><risdate>1984</risdate><volume>235</volume><issue>1</issue><spage>65</spage><epage>70</epage><pages>65-70</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><coden>CTSRCS</coden><abstract>The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstruction of serial sections. 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| ispartof | Cell and tissue research, 1984, Vol.235 (1), p.65-70 |
| issn | 0302-766X 1432-0878 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Biological and medical sciences Cell Nucleolus - ultrastructure Cell nucleus Cell structures and functions Chromatin - ultrastructure DNA - analysis Fundamental and applied biological sciences. Psychology Guinea Pigs Lymphocyte Activation Lymphocytes - drug effects Lymphocytes - ultrastructure Molecular and cellular biology Nucleolus Organizer Region - ultrastructure Phytohemagglutinins - pharmacology |
| title | The spatial organization of nucleolar DNA in phytohemagglutinin-stimulated lymphocytes of the guinea pig |
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