The spatial organization of nucleolar DNA in phytohemagglutinin-stimulated lymphocytes of the guinea pig

The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstructio...

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Veröffentlicht in:	Cell and tissue research 1984, Vol.235 (1), p.65-70
Hauptverfasser:	ANTEUNIS, A, POUCHELET, M, GANSMULLER, A, ROBINEAUX, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Nucleolus - ultrastructure Cell nucleus Cell structures and functions Chromatin - ultrastructure DNA - analysis Fundamental and applied biological sciences. Psychology Guinea Pigs Lymphocyte Activation Lymphocytes - drug effects Lymphocytes - ultrastructure Molecular and cellular biology Nucleolus Organizer Region - ultrastructure Phytohemagglutinins - pharmacology
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Zusammenfassung:	The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstruction of serial sections. The extended DNA filaments situated inside the fibrillar area originate from a large aggregation of heterochromatin, which is closely associated with the nucleolus, and from the perinucleolar shell of condensed chromatin. It is suggested that these two distinct regions of chromatin might be associated with different functions.
ISSN:	0302-766X 1432-0878
DOI:	10.1007/BF00213724