A method for electrophoretic characterization on polyacrylamide gel of circulating insulin immunoreactive substances

A method for electrophoretic characterization on polyacrylamide gel of circulating insulin immunoreactive substances

A method is described for determining the electrophoretic behavior of circulating insulin immunoreactive materials. A polyacrylamide immunoreactive pattern of serum can be determined on as little as 100 μU of insulin immunoreactive material. It is suggested that the utility of the method would be in...

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Veröffentlicht in:Analytical biochemistry 1971-04, Vol.40 (2), p.241-246
Hauptverfasser: Lazarus, Norman R., Gutman, Raul A., Recant, Lillian
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
Chromatography
Chromatography, Gel
Electrophoresis, Disc
Humans
Immune Sera - analysis
Insulin Antibodies - analysis
Methods
Radioimmunoassay
Swine
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Zusammenfassung:A method is described for determining the electrophoretic behavior of circulating insulin immunoreactive materials. A polyacrylamide immunoreactive pattern of serum can be determined on as little as 100 μU of insulin immunoreactive material. It is suggested that the utility of the method would be increased if it were coupled to a prior chromatographic separation of proinsulin like components from insulin by means of gel filtration. There is also considerable potential for use of this method as a preparative procedure for study of human circulating insulin components.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(71)90382-4