Detection of herpes simplex virus in clinical specimens by DNA hybridization
An assay to detect herpes simplex virus (HSV) DNA in clinical specimens has been developed. It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 × 104 plaque-forming units of cell-free HSV and as f...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 1983-06, Vol.1 (2), p.117-128 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Redfield, David C. Richman, Douglas D. Albanil, Sara Oxman, Michael N. Wahl, Geoffrey M. |
| description | An assay to detect herpes simplex virus (HSV) DNA in clinical specimens has been developed. It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 × 104 plaque-forming units of cell-free HSV and as few as four virus-infected cells. The assay has a sensitivity of 78% and a specificity of 100% compared to virus culture for the detection of HSV in swab specimens from genital lesions. No hybridization is observed with uninfected, varicella-zoster virus infected, or cytomegalovirus infected cells, and specimens from herpes zoster lesions are uniformly negative. While hybridization with a 32P-labeled probe is not optimally suited for routine diagnostic use, this report establishes the feasibility of using nucleic acid hybridization to detect HSV in clinical specimens. |
| doi_str_mv | 10.1016/0732-8893(83)90041-X |
| format | Article |
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It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 × 104 plaque-forming units of cell-free HSV and as few as four virus-infected cells. The assay has a sensitivity of 78% and a specificity of 100% compared to virus culture for the detection of HSV in swab specimens from genital lesions. No hybridization is observed with uninfected, varicella-zoster virus infected, or cytomegalovirus infected cells, and specimens from herpes zoster lesions are uniformly negative. 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It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 × 104 plaque-forming units of cell-free HSV and as few as four virus-infected cells. The assay has a sensitivity of 78% and a specificity of 100% compared to virus culture for the detection of HSV in swab specimens from genital lesions. No hybridization is observed with uninfected, varicella-zoster virus infected, or cytomegalovirus infected cells, and specimens from herpes zoster lesions are uniformly negative. While hybridization with a 32P-labeled probe is not optimally suited for routine diagnostic use, this report establishes the feasibility of using nucleic acid hybridization to detect HSV in clinical specimens.</description><subject>cytomegalovirus</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Herpes Simplex - microbiology</subject><subject>herpes simplex virus</subject><subject>Humans</subject><subject>Male</subject><subject>Nucleic Acid Hybridization</subject><subject>Plasmids</subject><subject>Simplexvirus - analysis</subject><subject>Species Specificity</subject><subject>varicella-zoster virus</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQx4MotVa_gUJOoofVyWazm70IpfUFRS8KvYU0O6GRfZm0xfrp3drSo54G5v-Y4UfIOYMbBiy9hYzHkZQ5v5L8OgdIWDQ9IH0mszwCyOCQ9PeWY3ISwgcAi_MEeqSX8liAhD6ZjHGBZuGamjaWztG3GGhwVVviF105vwzU1dSUrnZGlzS0aFyFdaCzNR2_DOl8PfOucN96U3FKjqwuA57t5oC8P9y_jZ6iyevj82g4iQyXchFZK0EIXqSm-yFJeC6YRGNYESPLLaZgpU26DRPaiFgUxmQzzXWW6SyNrRR8QC63va1vPpcYFqpywWBZ6hqbZVASpJSMw79GxtOYC5F1xmRrNL4JwaNVrXeV9mvFQG1oqw1KtUGpJFe_tNW0i13s-pezCot9aIe30--2OnY0Vg69CsZhbbBwvqOuisb9feAHtG2Ong</recordid><startdate>198306</startdate><enddate>198306</enddate><creator>Redfield, David C.</creator><creator>Richman, Douglas D.</creator><creator>Albanil, Sara</creator><creator>Oxman, Michael N.</creator><creator>Wahl, Geoffrey M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198306</creationdate><title>Detection of herpes simplex virus in clinical specimens by DNA hybridization</title><author>Redfield, David C. ; Richman, Douglas D. ; Albanil, Sara ; Oxman, Michael N. ; Wahl, Geoffrey M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-ff80553d6c5084439518ecc1d2e19fe60f8f48ec15ac525dcc7ba3a77a762f853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>cytomegalovirus</topic><topic>DNA, Viral - analysis</topic><topic>Female</topic><topic>Herpes Simplex - microbiology</topic><topic>herpes simplex virus</topic><topic>Humans</topic><topic>Male</topic><topic>Nucleic Acid Hybridization</topic><topic>Plasmids</topic><topic>Simplexvirus - analysis</topic><topic>Species Specificity</topic><topic>varicella-zoster virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Redfield, David C.</creatorcontrib><creatorcontrib>Richman, Douglas D.</creatorcontrib><creatorcontrib>Albanil, Sara</creatorcontrib><creatorcontrib>Oxman, Michael N.</creatorcontrib><creatorcontrib>Wahl, Geoffrey M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Redfield, David C.</au><au>Richman, Douglas D.</au><au>Albanil, Sara</au><au>Oxman, Michael N.</au><au>Wahl, Geoffrey M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of herpes simplex virus in clinical specimens by DNA hybridization</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>1983-06</date><risdate>1983</risdate><volume>1</volume><issue>2</issue><spage>117</spage><epage>128</epage><pages>117-128</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>An assay to detect herpes simplex virus (HSV) DNA in clinical specimens has been developed. It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 × 104 plaque-forming units of cell-free HSV and as few as four virus-infected cells. The assay has a sensitivity of 78% and a specificity of 100% compared to virus culture for the detection of HSV in swab specimens from genital lesions. No hybridization is observed with uninfected, varicella-zoster virus infected, or cytomegalovirus infected cells, and specimens from herpes zoster lesions are uniformly negative. While hybridization with a 32P-labeled probe is not optimally suited for routine diagnostic use, this report establishes the feasibility of using nucleic acid hybridization to detect HSV in clinical specimens.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6325080</pmid><doi>10.1016/0732-8893(83)90041-X</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0732-8893 |
| ispartof | Diagnostic microbiology and infectious disease, 1983-06, Vol.1 (2), p.117-128 |
| issn | 0732-8893 1879-0070 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | cytomegalovirus DNA, Viral - analysis Female Herpes Simplex - microbiology herpes simplex virus Humans Male Nucleic Acid Hybridization Plasmids Simplexvirus - analysis Species Specificity varicella-zoster virus |
| title | Detection of herpes simplex virus in clinical specimens by DNA hybridization |
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