Effect of divalent cations on the structure of human erythrocyte glucose 6-P dehydrogenase

Effect of divalent cations on the structure of human erythrocyte glucose 6-P dehydrogenase

The balance between the two active oligomeric forms of human G6PD, namely dimers and tetramers, is regulated by a number of environmental conditions: the dissociating effect displayed by high ionic strength and alkaline pH values is counteracted by several divalent cations resulting in formation of...

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Veröffentlicht in:Biochemical and biophysical research communications 1971-01, Vol.42 (2), p.159-165
Hauptverfasser: Bonsignore, A., Lorenzoni, I., Cancedda, R., Cosulich, M.E., De Flora, A.
Format: Artikel
Sprache:eng
Schlagworte:
Barium
Buffers
Calcium
Centrifugation, Density Gradient
Chemical Phenomena
Chemistry
Cobalt
Erythrocytes - enzymology
Glucosephosphate Dehydrogenase - blood
Humans
Hydrogen-Ion Concentration
Magnesium
Manganese
Osmolar Concentration
Polymers
Protein Binding
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Zusammenfassung:The balance between the two active oligomeric forms of human G6PD, namely dimers and tetramers, is regulated by a number of environmental conditions: the dissociating effect displayed by high ionic strength and alkaline pH values is counteracted by several divalent cations resulting in formation of tetramers. Binding of Mg 2+ ions to the protein determines association of two dimers through an allosteric transition.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(71)90082-9