Preparation of Haptens for Use in Immunoassays of Tetrahydro-11-deoxycortisol and Its Glucuronides

For the purpose of developing immunoassays of tetrahydro-11-deoxycortisol or its glucuronides, various haptenic derivatives were synthesized. The 3-hemisuccinate (15), 21-hemisuccinate (8), 3-hemiglutarate (16), 21-hemiglutarate (9) and glucuronides (19, 23, 25) of tetrahydro-11-deoxycortisol were prepared starting from 11-deoxycortisol 21-acetate (1). Then, antisera were elicited in two rabbits by immunization with the conjugate of tetrahydro-11-deoxycortisol 3-glucuronide (19) with bovine serum albumin. It was found that the antisera had binding affinities to enzyme-labeled antigens prepared from 15, 16 and 19 ; the binding was inhibited by the glucuronide (19) in the enzyme immunoassay procedure.